cdk9 d 7 (Santa Cruz Biotechnology)

Santa Cruz Biotechnology is a verified supplier

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Santa Cruz Biotechnology cdk9 d 7
$(A) Neurons were incubated for 3 h with ACBI (2.5 μM), and activity was induced by Bic + 4AP for 15 min. CoIP was then performed in neuronal lysates with <t>anti-CDK9.</t> CoIP samples were separated on a gel and blotted for other BAF subunits. SMARCA4 is used as a control to show ACBI efficiency. Spt6 is part of the elongation complex. (B) Neurons were incubated with MC180 or Thal-SNS-032 for 20 min or 3 h, respectively, followed by activity induction for 15 min with Bic + 4AP. Normalized Arc pre-mRNA levels were assayed and are displayed. (C) Neurons were treated as indicated and fractionated. The chromatin fraction was used, and anti-SMARCC2 coIP samples were electrophoresed and blotted for other BAF subunits. ARID1A is shown on a blot separate from the others. The ARID1A loading control (SMARCC2) is not displayed here to avoid duplication but is quantified in (D). (D and E) Quantification of (C) for ARID1A and SS18L1, respectively (normalized by SMARCC2). Quantification was performed as described for . (F) ChIP data to show SMARCC2 binding inside the Arc gene body 15 min after stimulation of wild type or neurons depleted of the subunit. (G) Quantified elongating RNA Pol II binding inside the Arc gene body, 15 min after stimulation, determined by ChIP with antibody against Rpb1-pSer2. (H) Quantified SMARCC2 binding inside the Arc gene body, after identical stimulation as in (G), determined by ChIP with antibody against SMARCC2. Gray dots represent biological replicates; error bars show SE of the mean. * p < 0.05, ** p < 0.01, and *** p < 0.001. One-way ANOVA was performed followed by Tukey’s post hoc test.$
Cdk9 D 7, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 409 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdk9+(d-7/Cdk9+Antibody/pmc11625021-9-0-3
Average 94 stars, based on 409 article reviews

cdk9 d 7 - by Bioz Stars, 2026-09

94/100 stars

Images

1) Product Images from "Activity-assembled nBAF complex mediates rapid immediate early gene transcription by regulating RNA polymerase II productive elongation"

Article Title: Activity-assembled nBAF complex mediates rapid immediate early gene transcription by regulating RNA polymerase II productive elongation

Journal: Cell reports

doi: 10.1016/j.celrep.2024.114877

$(A) Neurons were incubated for 3 h with ACBI (2.5 μM), and activity was induced by Bic + 4AP for 15 min. CoIP was then performed in neuronal lysates with anti-CDK9. CoIP samples were separated on a gel and blotted for other BAF subunits. SMARCA4 is used as a control to show ACBI efficiency. Spt6 is part of the elongation complex. (B) Neurons were incubated with MC180 or Thal-SNS-032 for 20 min or 3 h, respectively, followed by activity induction for 15 min with Bic + 4AP. Normalized Arc pre-mRNA levels were assayed and are displayed. (C) Neurons were treated as indicated and fractionated. The chromatin fraction was used, and anti-SMARCC2 coIP samples were electrophoresed and blotted for other BAF subunits. ARID1A is shown on a blot separate from the others. The ARID1A loading control (SMARCC2) is not displayed here to avoid duplication but is quantified in (D). (D and E) Quantification of (C) for ARID1A and SS18L1, respectively (normalized by SMARCC2). Quantification was performed as described for . (F) ChIP data to show SMARCC2 binding inside the Arc gene body 15 min after stimulation of wild type or neurons depleted of the subunit. (G) Quantified elongating RNA Pol II binding inside the Arc gene body, 15 min after stimulation, determined by ChIP with antibody against Rpb1-pSer2. (H) Quantified SMARCC2 binding inside the Arc gene body, after identical stimulation as in (G), determined by ChIP with antibody against SMARCC2. Gray dots represent biological replicates; error bars show SE of the mean. * p < 0.05, ** p < 0.01, and *** p < 0.001. One-way ANOVA was performed followed by Tukey’s post hoc test.$
Figure Legend Snippet: (A) Neurons were incubated for 3 h with ACBI (2.5 μM), and activity was induced by Bic + 4AP for 15 min. CoIP was then performed in neuronal lysates with anti-CDK9. CoIP samples were separated on a gel and blotted for other BAF subunits. SMARCA4 is used as a control to show ACBI efficiency. Spt6 is part of the elongation complex. (B) Neurons were incubated with MC180 or Thal-SNS-032 for 20 min or 3 h, respectively, followed by activity induction for 15 min with Bic + 4AP. Normalized Arc pre-mRNA levels were assayed and are displayed. (C) Neurons were treated as indicated and fractionated. The chromatin fraction was used, and anti-SMARCC2 coIP samples were electrophoresed and blotted for other BAF subunits. ARID1A is shown on a blot separate from the others. The ARID1A loading control (SMARCC2) is not displayed here to avoid duplication but is quantified in (D). (D and E) Quantification of (C) for ARID1A and SS18L1, respectively (normalized by SMARCC2). Quantification was performed as described for . (F) ChIP data to show SMARCC2 binding inside the Arc gene body 15 min after stimulation of wild type or neurons depleted of the subunit. (G) Quantified elongating RNA Pol II binding inside the Arc gene body, 15 min after stimulation, determined by ChIP with antibody against Rpb1-pSer2. (H) Quantified SMARCC2 binding inside the Arc gene body, after identical stimulation as in (G), determined by ChIP with antibody against SMARCC2. Gray dots represent biological replicates; error bars show SE of the mean. * p < 0.05, ** p < 0.01, and *** p < 0.001. One-way ANOVA was performed followed by Tukey’s post hoc test.

Techniques Used: Incubation, Activity Assay, Control, Binding Assay

Figure Legend Snippet: KEY RESOURCES TABLE

Techniques Used: Recombinant, Plasmid Preparation, Magnetic Beads, One Step RT-PCR, SYBR Green Assay, Mass Spectrometry, Software

Blocking Assay:

Article Title: Inhibition of SF3B1 affects recruitment of P-TEFb to chromatin through multiple mechanisms
Article Snippet: .. Blocking solution was removed and coverslips were incubated with primary antibodies (Rabbit anti-G3BP1 (17798, Cell Signaling), 1:500; Mouse anti-CDK9 (D-7) (sc-13130, Santa Cruz Biotechnology), 1:500; Rabbit anti-SC35 (SRSF2) (ab204916, Abcam), 1:100) diluted in blocking solution overnight at 4°C. ..

Incubation:

Biomarker Discovery:

Article Title: ERK-mediated NELF-A phosphorylation promotes transcription elongation of immediate-early genes by releasing promoter-proximal pausing of RNA polymerase II
Article Snippet: 3 n atu re p o rtfo lio | rep o rtin g su m m ary M a rch 2021 Antibodies Antibodies used Validation The following primary antibodies were used: anti-NELF-A G-11 (Santa Cruz Biotechnology, sc-365004) (1:3000) anti-NELF-D C-10 (Santa Cruz Biotechnology, sc-393972) (1:2000) anti-NELF-E F-9 (Santa Cruz Biotechnology, sc-377052) (1:2000) anti-ERK1/2 C-9 (Santa Cruz Biotechnology, sc-514302) (1:1000) anti-ERK1 K-23 (Santa Cruz Biotechnology, sc-94) (1:1000) anti-Myc 9E10 (Santa Cruz Biotechnology, sc-40) (1:1000) anti-GST B-14 (Santa Cruz Biotechnology, sc-138) (1:1000) anti-HA F-7 (Santa Cruz Biotechnology, sc-7392) (1:1000) anti-CyclinD1 A-12 (Santa Cruz Biotechnology, sc-8396) (1:1000) anti-Pol II 8WG16 (Santa Cruz Biotechnology, sc-56767) (1:2000) anti-Pol II CTD4H8 (Santa Cruz Biotechnology, sc-47701) (1:2000) anti-CDK9 D-7 (Santa Cruz Biotechnology, sc-13130) (1:2000) anti-Elk1 E-5 (Santa Cruz Biotechnology, sc-365876) (1:1000) anti-Myc-Tag 9B11 (Cell Signaling Technology, 2276) (1:1000) anti-Phospho-ERK1/2 (Cell Signaling Technology, 9101) (1:1000) anti-Phospho-p90RSK T573 (Cell Signaling Technology, 9346) (1:1000) anti-Phospho-Rpb1 CTD-Ser5 D9N5I (Cell Signaling Technology, 13523) (1:2000) anti-RSK1/RSK2/RSK3 32D7 (Cell Signaling Technology, 9355) (1:1000) anti-Phospho-Rpb1 CTD-Ser2 E1Z3G (Cell Signaling Technology, 13499) (1:1000) anti-c-Fos 9F6 (Cell Signaling Technology, 2250) (1:1000) anti-HA 3F10 (Roche, 11867423001) (1:1000) anti-Flag M2 (Sigma, F1804) (1:1000) anti !-Actin (FUJIFILM Wako, 010-27841) (1:1000) anti-His-tag (Medical & Biological Laboratories, D291-3) (1:1000) anti-MPM2 (anti-phospho-SP or TP) (Millipore, 05-368) (1:1000) anti-NELF-A (Protein tech, 10456-1-AP) (1:3000) anti-NELF-B (Protein tech, 16418-1-AP) (1:2000) anti-GADD45B (Cloud-Clone, PAL535Hu01) (1:1000) anti-DICE1/INST6 (Santa Cruz Biotechnology, sc-376524) (1:1000) anti-PP2Ac (BD, 610555) (1:1000) anti-RAS (G12V mutant) GeneTex GTX132694 (1:1000) anti-BrdU (Monoclonal antibody from mouse, BMG 6H8 IgG1; Sigma, 5-Bromo-2#-deoxy-uridine Labeling and Detection Kit I, 11296736001) The following secondary antibodies were used : anti-mouse IgG-horse radish peroxidase (HRP) antibody (NA931, Cytiva) (1:5000) anti-rabbit IgG-HRP antibody (NA934, Cytiva) (1:2500) Alexa-Fluor 488 goat anti-mouse IgG (A-11029, Molecular Probes) (1:2000) All antibodies used in this study were purchased from commercial vendors. .. Validation of antibodies used in current study is described in technical data sheets provided by manufacturers websites: anti-NELF-A G-11 (Santa Cruz Biotechnology, sc-365004): https://www.scbt.com/p/nelf-a-antibody-g-11?requestFrom=search anti-NELF-D C-10 (Santa Cruz Biotechnology, sc-393972) (1:2000): https://www.scbt.com/p/nelf-d-antibody-c-10? requestFrom=search anti-NELF-E F-9 (Santa Cruz Biotechnology, sc-377052) (1:2000): https://www.scbt.com/p/nelf-e-antibody-f-9?requestFrom=search anti-ERK1/2 C-9 (Santa Cruz Biotechnology, sc-514302) (1:1000): https://www.scbt.com/p/erk-1-2-antibody-c-9?requestFrom=search anti-ERK1 K-23 (Santa Cruz Biotechnology, sc-94) (1:1000): https://www.scbt.com/p/erk-1-antibody-k-23?requestFrom=search anti-Myc 9E10 (Santa Cruz Biotechnology, sc-40) (1:1000): https://www.scbt.com/p/c-myc-antibody-9e10?requestFrom=search anti-GST B-14 (Santa Cruz Biotechnology, sc-138) (1:1000): https://www.scbt.com/p/gst-antibody-b-14?requestFrom=search anti-HA F-7 (Santa Cruz Biotechnology, sc-7392) (1:1000): https://www.scbt.com/ja/p/ha-probe-antibody-f-7?requestFrom=search anti-CyclinD1 A-12 (Santa Cruz Biotechnology, sc-8396) (1:1000): https://www.scbt.com/p/cyclin-d1-antibody-a-12? requestFrom=search anti-Pol II 8WG16 (Santa Cruz Biotechnology, sc-56767) (1:2000): https://www.scbt.com/ja/p/pol-ii-antibody-8wg16? requestFrom=search anti-Pol II CTD4H8 (Santa Cruz Biotechnology, sc-47701) (1:2000): https://www.scbt.com/p/pol-ii-antibody-ctd4h8? requestFrom=search anti-CDK9 D-7 (Santa Cruz Biotechnology, sc-13130) (1:2000): https://www.scbt.com/ja/p/cdk9-antibody-d-7?requestFrom=search anti-Elk1 E-5 (Santa Cruz Biotechnology, sc-365876) (1:1000): https://www.scbt.com/p/elk-1-antibody-e-5?requestFrom=search anti-Myc-Tag 9B11 (Cell Signaling Technology, 2276) (1:1000): https://www.cellsignal.com/products/primary-antibodies/myctag-9b11-mouse-mab/2276 anti-Phospho-ERK1/2 (Cell Signaling Technology, 9101) (1:1000): https://www.cellsignal.com/products/primary-antibodies/phosphop44-42-mapk-erk1-2-thr202-tyr204-antibody/9101 anti-Phospho-p90RSK T573 (Cell Signaling Technology, 9346) (1:1000): https://www.cellsignal.com/products/primary-antibodies/ ..

other:

Article Title: Evolutionary analysis reveals the role of a non-catalytic domain of peptidyl arginine deiminase 2 in transcriptional regulation
Article Snippet: Rabbit polyclonal anti-CDK9 (D-7) , Santa Cruz Biot. , SC-13130.

Article Title: Activity-assembled nBAF complex mediates rapid immediate early gene transcription by regulating RNA polymerase II productive elongation
Article Snippet: CDK9 (D-7) , Santa Cruz Biotechnology , Cat#: sc-13130 Mouse mAb RRID: AB_627245.

Immunoprecipitation:

Article Title: BRD4 regulates Aurora B kinase activity
Article Snippet: Purified recombinant PTEFb (CDK9) was purchased from Sigma-Millipore. .. Antibodies used for either immunoprecipitation or immunoblotting were anti-BRD4 mouse monoclonal antibody (Sigma; AMAB90841), anti-Aurora B rabbit polyclonal antibody (Abcam; ab2254), Anti-His probe (SantaCruz biotech H-3: sc-8036), anti-Histone H3 (Cell Signaling), anti-Pan-Acetyl Histone H3 (Millipore), Anti-Histone H3 pS10 (SantaCruz biotech; sc-8656-R), Anti-Histone H3pS28 (GeneTex, GTX128953), anti-MYC and anti-MYC pThr58 (Abcam, Y69: ab32072 and ab185655), anti-CDK9 (SantaCruz biotech D-7: sc-13130), anti-CDK9 pT186 (Sigma; SAB4504223), anti-KIF2C/MCAK pS95 (LSBio; LS-C381163), anti-JNK and anti-pJNK (SantaCruz biotech D-2: sc-7345 and G-7; sc-6254) and anti-beta tubulin (Abcam, ab6046). anti-BRD4 pThr1212 was custom-made and will be shared upon request. .. Anisomycin (Goldbio; A-580-25) was dissolved in dimethyl sulfoxide (DMSO); JNK inhibitor D-JNKI-1 (MedChemExpress; HY-P0069) and Indole-3-acetic acid/Auxin (Sigma; I5148) was dissolved in water to make stock solutions.

Western Blot:

Article Title: Resistance of estrogen receptor function to BET bromodomain inhibition is mediated by transcriptional coactivator cooperativity.
Article Snippet: The bromodomain and extraterminal domain (BET) family of proteins are critical chromatin readers that bind to acetylated histones through their bromodomains to activate transcription.. Here, we reveal that bromodomain inhibition fails to repress oncogenic targets of estrogen receptor because of an intrinsic transcriptional mechanism.. While bromodomains are necessary for the transcription of many genes, bromodomain-containing protein 4 (BRD4) binds to estrogen receptor binding sites and activates transcription of critical oncogenes such as MYC, independently of its bromodomains.

Review